SoftMax Pro Software enables users to quickly set up analysis of cell counts via a simple point and click user interface. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 3 Figures from 612 Publications Promega. . AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. 1. 100 fmol 384-well (75 µL) 0. After 2 hours of treatment cells were harvested by centrifugation and cell pellets were lysed in RIPA buffer (20mM Tris-Cl, 150mM NaCl, 0. Ease of use Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. More Product Information. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. HTRF IP standard curve. SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. The ELISA plates were read at an absorbance of 450 nm on a SpectraMax i3x Plate Reader using Softmax Pro 7. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThis characterization of nanoparticle and its surface interactions can be detected using the SpectraMax i3x Multi-Mode Microplate Reader and the Spectral Optimization Wizard in SoftMax Pro Software. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. ZERO BIAS - scores, article reviews, protocol conditions and moreStructured Review. In this product video, see how the SpectraMax M5 quickly reads samples. . The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. SpectraDrop Abs Protein Quant (i3-i3x)Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. 2. 99 to $1999. . The MiniMax cytometer with SoftMax® Pro Software can be used to image and analyze a variety of cells. \r Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48 AM The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. A: percent GFP-positive cells calculated using the classification feature in the software. The microplate was then placed in a SpectraMax i3x reader and incubated at 37°C for the duration of the kinetic measurement. Ribonuclease (RNase) R digestion assay For identifying the stabilization of circVRK1, 2 μg isolated RNA from HBMECs was digested by RNase R (3 U/μg; RNR07250, Geneseed, Guangzhou,. Example: [Drawer]. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. Both Microplate Optimization and Read Height Adjustment were. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Open the catalog to page 2. 1、卡盒式模块和细胞成像模块的构架检测功能（如FP、TRF、HTRF、AlphaScreen、ScanLater Western Blot等）卡盒模块化设计，仅通过插入卡盒就能可. SpectraMax Quant dsDNA Assay Kits. The Curcumin binding to p53Y220C was also measured using the emission maxima of Curcumin at 495 nm after. 1 software Download page. SpectraMax® i3x. i3. Figure 1. 2 software (Stat-Ease) to identify factors that have significant effects on cell growth or IgG production, either alone or in combination with. ”Figure 3: Sensitivity of the SpectraMax i3x reader in detecting increasing dilutions of the MycoAlert Assay Control. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. 因为是导师第一任学生，没有师兄师姐，加上本人悟性也差，小小pcr花了半年时间才终于有点样子。因为经历过，所以懂得，故而分享之，希望能给到屏幕前的你一点点帮助! 如有失误，欢迎指正~最后，不要忘了一键三联哟，谢谢大家！, 视频播放量 6293、弹幕量 2、点赞数 122、投硬币枚数 55、收藏. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. , Fisher Scienti fi c, cat. SpectraMax MiniMax 300 细胞成像系统 为 SpectraMax i3x 多功能微孔板检测平台而打造！ • • • • 主要特点 系统全面兼容细胞成像 及微孔板检测技术 独特无标记分析技术无需 对细胞染色即可进行细胞 计数和贴壁度的计算 具有多种检测功能可对 细胞活力及毒性进行全. Note: Additional readers with similar performance are listed. With optimized reagents, option to operate the readers. Average 98 stars, based on 1 article reviews. com The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Cet appareil ISM est confomre à la norme NMB-001 du Canada. It allows you to explore cellular pathways and protein activation and expression in one system. Nuclear Receptor. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. 306 plate air cal. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 58% for the detection module and 2. The SpectraMax® DuoLuc™ Reporter Assay Kit enables highly sensitive quantitation of both firefly and Renilla luciferases in mammalian cells at a great price. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Keywords: htrf spectramax detection cartridge, spectramax i3, spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf. Danaher Inc spectramax i3x. Shake intensity. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Clamp for injector 1 is on the left and clamp for injector 2 is on the right. Description 1. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Published Dec 16, 2020 | Updated Oct 10, 2023. Learn how you can perform flash-based applications, such as dual luciferase and ATP assays, with high precision and accuracy using this cartridge. The corrected absorbance is within 5% of the. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Explore PDF for more details. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. The dynamic range of the assay is about 3. In SoftMax® Pro, the following Automix settings are available for the SpectraMax i3/i3x Instrument: Intensity : Low, Medium, or High. The solutions were thoroughly mixed and then incubated in the dark at 20°C for 2 h. . Unlimited breadth of. EUROPE. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. モジュールを追加してアップグレードすることにより、ウェスタンブロットやイメージング、試薬インジェクタを用いた高速カイネティック測定を行う. to View Figures. 303 unable to cal dark current. SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax. Danaher Inc spectramax i3x. application modules • Multi-mode microplate reader and imaging system • Wide dynamic range • StainFree™ cell counting technology • Library of user-configurable applications • Advanced curve fitting and statistical analysis • Cell confluence and cell viabilitySoftMax® Pro 7. 5 Figures from 202 Publications Thermo Fisher Scientific. Description 1. SpectraMax M5. Figure 1. Catalog excerpts. 305 reference level saturation. . The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring Download SoftMax® Pro 7. Spectranet customer portalThe SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. Shaking and incubations were performed using an on-deck orbital shaker. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. R8362. 305 reference level saturation. ClonePix 2 Mammalian Colony Picker; QPix Microbial Colony Pickers; CloneSelect Imager; DispenCell Single-cell Dispenser; 3D. to View Figures. . 0´ 105 dsD NA (p g/ wel ) R F UFluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). The Results. Spectranet customer portal. to View Figures. . Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick inspection of cells and provides insight into phenotypic changes that are important for understanding cytotoxicity, cell proliferation, and protein expression. 1. SpectraMax i3Instrumenttovisuallyinspectyoursampleandtoruncell-basedassaysat cellularorwhole-cellresolution. iD5. User Manuals, Guides and Specifications for your Molecular Devices SpectraMax i3x Measuring Instruments. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. . Bioz Stars score: 98/100, based on 1 PubMed citations. g. STAKMAX. . Spectramax I3x, supplied by Danaher Inc, used in various techniques. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. Modularity does provide some measure of forward vision into upcoming or future experimental needs. VAL014, right table) assays on the SpectraMax i3x reader are shown below. Download SoftMax® Pro 7. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. 5mM EDTA, 1% NP40,Explore a wealth of applications with the future-ready SpectraMax i3x. Amplitude : 1. 020 fmol 0. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. 6% for the on-board optics (monochromators). With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. viii SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual Conventions Used in this Manual The names of keys that appear on the SPECTRAmax 340PC control panel are shown in boxed Helvetica type. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. Quantification of NETs was performed with the SpectraMax i3x microplate-reader (Molecular Devices, United Kingdom). Data were analyzed with SoftMax ® Pro Software and graphed using a 4-parameter curve fit. Quantiferon-TB Gold in Tube Kit. 99. GFP expression imaged on the cytometer. SpectraMax® ABS and ABS Plus reader support; ü. At each time point, the samples were shaken and fluorescence was measured for SEP (488-nm excitation and 530-nm emission) and. 4. . It can be upgraded with. . Contact Us Phone: +1. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Restart the system. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. 030 fmol. 312 gain calibration failed. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax i3x Multi-Mode microplatereader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging and Fast Kinetics with Injectors. The protocols are also available on the SoftMax Pro Software protocol sharing. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. ISM Equipment Classification (Group 1, Class A) This equipment is. 1. Check software compatibility with Windows 10 and locate your software installer. The TUNE detection cartridge uses a breakthrough monochromator design that enables researchers to easily optimize their. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. +44-118-944-8000. . Version 7. This update is applicable for users of the SpectraMax® i3x Multi-Mode Detection Platform and SpectraMax® Paradigm® Multi-Mode Microplate Reader with the ScanLater Western Blot (WB) Detection Cartridge and the SpectraMax® iD5 Multi-Mode Microplate Reader: SMP 7. StackMax Microplate Handling System. ü; ü. 5. The SoftMax Pro Software provides integrated instrument control. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with. Molecular Devices推出的SpectraMax® i3x 多功能酶标仪除了具有全波长的光吸收、荧光、化学发光和FRET检测功能以外，还兼容了客户端模块化升级功能，用户可以根据需要任何时间随意升级至荧光偏振FP、HTRF、AlphaScreen、Western Blot、上转发光检测、细胞成像和带有注射器. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. i3x. detection cartridge LUM96: 59. SpectraMaxParadigmMulti-ModeDetectionPlatformUserGuide 4 5014038F Chapter5:ReadModesandReadTypes 71 SupportedReadTypes 71 AbsorbanceReadMode 74 FluorescenceIntensityReadMode 79the SpectraMax Paradigm Multi-Mode Detection Platform User Guide. Molecular Devices Software. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Microplate optimization should be performed the first time the assay is run, and subsequently for . With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. The treatments were. Download PDF. The SpectraMax® i3x reader allows you to. Absorbance Fluorescence (top/bottom read) Luminescence (top read) Time-Resolved Fluorescence (top read). SeeSpectraMax MiniMaxImagingCytometeronpage85. Genscript cPass SARS-CoV-2 Neutralization Antibody Detection. Molecular Devices SpectraMax i3x Multi-Mode Microplate Platform. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Monday to Friday 7am - 5pm Pacific Time. 0. The spectra analysis and K d were determined using Sigmaplot 14. We would like to show you a description here but the site won’t allow us. Page 28 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 4. 1. Bioz Stars score: 98/100, based on 1 PubMed citations. No accessories are needed and SoftMax® Pro. All the readers were operated using SoftMax® Pro Software, which also has built-in data analysis and curve-fitting for automatic calculation of EC 50. Eight different automated tests are provided: • Absorbance accuracy (linearity) • Precision (reproducibility) • Stray light • Wavelength accuracy • Wavelength repeatability SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Description 1. . 더 많은. The CellTiter-Glo assay from Promega uses luciferase. SpectraMax i3x Multi-Mode Microplate Reader. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles) readers. Explore Multi-Mode Validation Plate that confirms instrument performance for spectramax i3, i3x, Paradigm system & FilterMax Reader. The calculated LOQ values were 0. Bioz Stars score: 98/100, based on 1 PubMed citations. #0200-7009) Contact our support team with your questions regarding Molecular Devices products and applications. Read height should be optimized for . Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. Align the two slots on the bottle holder with the two pegs on the rail. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology Measure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. Background noise is reduced by the reader’s cooled PMT, enabling increased sensitivity and dynamic range. SoftMax Pro. SpectraMax® i3x Multi-Mode Detection Platform; SpectraMax® i3 Multi-Mode Detection Platform; SpectraMax® ID3 Multimode Microplate reader; SpectraMax® ID5 Multimode Microplate reader; SpectraMax® Paradigm® Multi-Mode Microplate Reader; SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. . . Spectramax I3x, supplied by Danaher Inc, used in various techniques. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. Instrument. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. This technique allows for quality control and assurance when performing surface functionalization of nanoparticles. SpectraMax Microplate Readers. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. Download PDF. SpectraMax Paradigm Platform in conjunction with AlphaLISA provides a powerful tool to screen novel therapeutics in a pathologically relevant, endothelial cell-based model. The experiments were performed in triplicates and the spectral reading was recorded using Spectramax i3x microplate reader. Fractional occupancy Corrected BRET ratio NanoBRET. Replicate values were averaged and imported into Design-Expert 8. The SpectraMax i3x is a multi-mode microplate reader that measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. ; The top 3 importers of Spectramax are India with 365. ImageXpress Confocal HT. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. . Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. 313 reference gain check fail. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellEvery SpectraMax ® Paradigm Detection Cartridge starts with an application optimized excitation energy source either in the form of a long life, high powered LED, xenon flash lamp, or high powered laser. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. The SpectraMax M4 reader had identical performance specifications to the SpectraMaxLuminescence was read on the SpectraMax i3x reader and the SpectraMax L reader. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. china@moldev. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. 99 to $1999. See SpectraMax® Microplate Reader User Manual Download Page. Subsequently, 100 μL of the substrate solution (2 mM of p-nitrophenyl butyrate in dimethylformamide) was added into the mixture, and incubated at 37°C for 15 min. ZERO BIAS - scores, article reviews, protocol conditions and more. The TRF intensities measured are proportional to the level of extracellular acidification in each sample. Insert the snorkel into the bottle and then insert the snorkel into the snorkel clamp. The chamber. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. 58% for the detection module and 2. o For example: Ex: 485/Em 525/Cutoff 515 4. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. 314 low lamp level warning. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. 304 signal level saturation. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves. 7 Figures from 1126 Publications. Remove the transport bracket and store the retaining screws in the bag that held the 3. Immunofluorescence of In Vitro LPS-Stimulated Neutrophils. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any application or budget. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. Reporter Assay. )wideand65. SpectraMax i3, i3x, and iD5 readers. 1. Bioz Stars score: 98/100, based on 1 PubMed citations. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely. SpectraMax ® iD3 and iD5 Multi-Mode . Finally, their fluorescence was measured with 365-nm excitation and 450-nm emission filters using a microplate fluorometer (SpectraMax i3x, Molecular Devices, San Jose, CA, USA) (Saiya-Cork et al. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Tip: MCF-7 cells have a polygonal morphology with irregular dimensions, and they have a habit of clumping into large aggregates. Overview of Western Blot Cartridge. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. Actual shake speed is based on the microplate format. SpectraMaxi3Multi. Figure 1. We would like to show you a description here but the site won’t allow us. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors; It allows you to explore cellular pathways and protein activation and expression in one system;In this application note, we describe how the SpectraMax ® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. GM3000 Register or Sign In. Microplate Reader Shelf. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. SpectraMax i3x; SpectraMax iD3 / iD5; SpectraMax M Series; SoftMax Pro Software; CELLULAR IMAGING. User-upgradeable detection modules allow you to expand the capabilities of your microp. TR-FRET ratios were calculated by dividing the RFU value for the 665-nm reading by the RFU value for the 616-nm reading, and the resulting numbers were multiplied by 1000. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. . 2. View the Molecular Devices website. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. . BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. . . techsupport. Bioz Stars score: 98/100, based on 1 PubMed citations. SpectraMax i3x, onboard luminescence 121. Database contains 1 Molecular Devices SpectraMax i3x Manuals (available for free online viewing or downloading in PDF): Installation manual . HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles). Spectramax I3x, supplied by Danaher Inc, used in various techniques. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. Note: Additional readers with similar performance are listed. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Serial number 33 900 01-3387. . . Supported Windows Operating System: See release notes. Citation 2002). ValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. Description 1. ValitaTiter Plus settingsContact our support team with your questions regarding Molecular Devices products and applications. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. For wavelengths in the UV range above 220 nm,. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. Title: Fluorescence Intensity SpectraMax Paradigm or i3 Detection Cartridge | Molecular Devices Author: Molecular Devices, LLC Subject: Explore this datasheet to know more about Fluorescence Intensity Detection Cartridges for SpectraMax Paradigm and i3x, which provide speed, sensitivity, and flexibility & uncompromising performance for either top or. Facilities. Methods. . . SpectraMax Paradigm Cisbio HTRF Detection Cartridge User Guide 5008539 A 5 Laser Light Laser Specifications The SpectraMax® Paradigm® Multi-Mode Detection Platform is rated a Class 1 Laser Product because it houses one or more Laser Modules whereas the laser light is not accessible. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Danaher Inc spectramax i3x. . Molecular Devices. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. . KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyThe SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. The SpectraMax i3x reader offers a modular design allowing the addition of FP detection to the instrument in just minutes. Negative samples contained only MycoAlert Assay Buffer. Summary of Contents for Molecular Devices SpectraMax M2. . i3x Multi-Mode Microplate Reader. (3) Molecular Devices, SpectraMax i3x, Microplate Readers (2) 2022 (1) 2020 Invetech Korus KRS-100 Elutriation & Washing Systems (3) 2021 ThermoFisher Scientific, invitrogen, CytKick Max AutoSampler w/ Attune NxT: Acea NovoCyte Flow Cytometer: Appliedbiosystems, QuantStudio 7 Flex, Real-Time PCR SystemThe SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. SpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. +44 118 944 8000. Eliminate time-dependent substrate addition steps. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. The microplate was then placed in a SpectraMax i3x reader and incubated at 37°C for the duration of the kinetic measurement. Detection cartridge modularity allows for easy addition of new read modes within minutes without. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Homogeneous and eterogeneous microplate assays can be performed in one flexible system. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. For more information on the SpectraMax i3x Multi-Mode Microplate Reader System, please click here. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideProducts : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. application modules • Multi-mode microplate reader and imaging system • Wide dynamic range • StainFree™ cell counting technology • Library of user-configurable applications • Advanced curve fitting and statistical analysis • Cell confluence and cell viability Introduction. ; SoftMax® Pro 7. . As per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. 58% for the detection module and 2. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. 2 only) SpectraMax M-series Microplate readers. 100 fmol 1536-well (8 µL) 0. Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. Sufficient for 1000 reactions. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes With these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay.